Desarrollo de andamios biodegradables mediante separación de fases con una potencial aplicación en Ingeniería Tisular

Abstract

The present work focuses on the development of scaffolds based on collagen and chitosan using different processing methods derived from the fabrication of hydrogels and freeze-drying. The scaffolds were produced using 3 different processes and with 1 wt.% of two different biopolymers (collagen and/or chitosan). In order to evaluate the composition and manufacturing method of scaffolding, their mechanical properties were evaluated to determine: (1) their rheological properties through strain and frequency sweeps, (2) their porosity through an analytical model and a mercury porosimeter, and (3) their structure and fiber arrangement by scanning electron microscopy. The results obtained reveal that the properties of the scaffolds were strongly dependent on both the proportion of the raw materials used and the processing method. Furthermore, it was found that a synergic effect occurs when collagen and chitosan in a 1:1 ratio are used in their composition, and also when temperature is increased, thus the solubilization of biopolymers and their interaction during mixing is favored.